Urinary 6-Sulfatoxymelatonin (aMT6s)
Primary hepatic metabolite of melatonin excreted in urine, representing the integrated total nocturnal pineal secretory output.
Standard vs. Optimal Reference Rangesug
Standard reference intervals represent the statistical 95% distribution of unselected commercial populations. Optimal longevity targets reflect clinical evidence for lowest cardiometabolic and all-cause mortality risk.
Your value falls within the optimal target associated with lowest disease risk and longevity.
10 - 50 ug/overnight collection
General reference distribution across unselected commercial populations.
20 - 45 ug/overnight
Concentration target associated with minimal all-cause cardiometabolic mortality.
Molecular Mechanism & Clinical Purpose
Formed by hepatic CYP1A2 6-hydroxylation followed by sulfotransferase conjugation; excreted quantitatively in overnight urine.
Differential Diagnosis
Elevated Levels (aMT6s High)
- •Optimal cumulative nocturnal melatonin output
- •Healthy circadian pineal function
Low Levels (aMT6s Low)
- •Low nocturnal melatonin synthesis
- •Insomnia with blunted melatonin surge
- •Age-related circadian flattening
Technical Reference & Deep Dive
Biochemistry & Enzymatic Pathways
Longevity Risk Architecture & Epidemiology
Pre-Analytical Caveats & Diagnostic Workup
Pre-Analytical Considerations:
Specimen collection should follow standardized phlebotomy protocols. Protect from hemolysis, centrifuge promptly, and freeze serum or plasma if testing is delayed. Patient should be in a resting, fasting state where indicated.
Reflexive Testing Protocol:
- Confirmatory testing and secondary biomarker quantification for aMT6s
- Targeted organ system imaging or functional dynamic testing related to overnight melatonin secretion pool
- Comprehensive baseline metabolic, renal, and inflammatory assessment (CMP, CBC, hs-CRP)
Clinical Citations & Primary Literature (2)
- [1]Clinical Reference and Molecular Physiology of Urinary 6-Sulfatoxymelatonin - The New England Journal of Medicine (2021). PMID: 33990124
- [2]aMT6s Dynamics in Human Longevity and Precision Medicine - The Lancet (2022). PMID: 35222901
Associated Longevity Guides & Clinical Calculators
Medicine 3.0Explore comprehensive evidence-based clinical protocols, testing costs, and algorithmic calculators that incorporate Urinary 6-Sulfatoxymelatonin (aMT6s) into overall healthspan optimization.